1. To ensure the pure culture of strains, be careful not to use bacterial strains contaminated with bacteria, not to connect cultures at close range.
2. Strictly control the passage times of strains, reduce mechanical damage, and ensure the viability of strains.
3, appropriate cryopreservation strains, low-temperature strains such as mushrooms, mushrooms, etc., at 4 °C conducive to the preservation of the vitality of mycelium, high-temperature strains such as straw mushroom at 16 °C conducive to preservation of the vitality of mycelium.
4. Avoid multiple passages in a single medium; strains should not be used for too long, and overage bacteria will appear to age, while aging and degradation are organically linked, and viable strains are prone to degradation.
5, bacteria should be rejuvenated regularly, in the appropriate temperature, suitable pH, adequate oxygen, no bacteria culture conditions.
6. Spores are separated every year. Sexual reproduction is used to find good strains, and tissue separation is used to consolidate the genetic characteristics of good strains.
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