A large body of evidence indicates that microRNAs ( miRNAs ) are abundantly present in the liver and play an important role in liver development, regeneration and disease development. In order to better promote liver disease and marine life research, Professor Lu Zhengbing from Zhejiang University of Technology recently led his team to conduct in-depth research on miRNAs in the liver of the striped bamboo shark . The research results were published in the July issue of BioMed Research International .
Although the genome sequencing of the striped bamboo shark has not been completed, the recently proposed callerhinchus milii genome is an ideal model for the cartilage fish genome. Therefore, the researchers used the Y. japonicus genome to predict the hairpin structure of pre-miRNA . Through small RNA sequencing, a total of 91 known miRNAs and 166 putative candidate miRNAs were identified for the first time in the striped bamboo shark ( small RNA sequencing service was completed by Lianchuan Bio ). Through target prediction and GO analysis, 91 In the known miRNA screened out 46 of miRNAs, in particular cell proliferation and growth-related miRNAs.
The researchers further analyzed the expression of miRNAs and miRNAs in the striped bamboo shark by RT-PCR . Selected differentially expressed genes in normal and regenerating liver tissue, obtained by identifying sequence of ten.
This study is the first comprehensive report on cartilage miRNAs . A large number of new miRNA sequence information was added to the existing database of the striped bamboo shark , and laid a foundation for further study of the function of miRNAs in the liver development of the striped bamboo shark. It contributes not only to fish but also to liver miRNA research in other species .
* Conger Lu, et al. (2013) Study of MicroRNAs Related to the Liver Regeneration of the Whitespotted Bamboo Shark, Chiloscyllium plagiosum . BioMed Research International . [ article ]
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