First, low temperature hardening seedlings. Low-temperature hardening seedlings can significantly enhance the low-temperature tolerance of vegetable seedlings, which is beneficial to the early emergence of seedlings after planting. The low-temperature hardening seedlings are generally carried out 7 to 10 days before the vegetable is planted and cannot be used for too long. Nursery bed temperature during the day in 15 ~ 20 °C, reduced to 8 ~ 12 °C at night, in order to improve the cold resistance of seedlings. The temperature of seedbed during low-temperature hardening cannot be too low, otherwise it will affect the normal physiological function of the root system, and prone to aging and stiff seedlings, affecting normal growth.
Second, advance sheds. In order to rejuvenate the seedlings as soon as possible after planting the vegetable seedlings, the greenhouse is required to reach an optimum temperature, especially for vegetables such as tomatoes, eggplants, cucumbers, etc. These vegetables have higher requirements for the temperature and temperature of the ground, requiring 5 to 10 The ground temperature of centimeters must reach above 15°C, and the minimum temperature must not fall below 10°C in order to grow normally. Therefore, 10 days before the planting of vegetable seedlings, the greenhouse should be covered with a plastic film to close the greenhouse and raise the temperature.
Third, timely planting. In early spring, the temperature is unstable, and it is prone to cold weather such as plunge cold, strong wind cooling, and snow freezing. Vegetable seedlings must pay attention to listening to the weather forecast before planting, and require no cooling and cloudy snow weather within 5 to 7 days. .
Fourth, strengthen insulation. After the planting of seedlings, we must strengthen the insulation work to promote the seedlings. Within 3 to 5 days after planting, the greenhouses should be enclosed and the winds should be released. The daytime temperature is maintained between 28 and 30°C and the nighttime temperature is maintained above 20°C. In case of strong winds and cool weather, cover the insulation at night to prevent excessive heat dissipation at night, causing the temperature of the greenhouse to fall too quickly. In addition, the laying of crop stalks in the operation line can not only increase the ground temperature, but also reduce the humidity in the shed, which is conducive to the healthy growth of vegetables.
Magnetic Bead Nucleic Acid Extraction Reagent
The magnetic bead method nucleic acid extraction kit is a high-tech product that combines biological science and nanomaterial science. It is a major breakthrough in my country's nucleic acid extraction and purification technology. . Magnetic bead method for nucleic acid extraction has incomparable advantages over traditional DNA extraction methods, which are mainly reflected in: 1. It can realize automatic and large-scale operation. At present, there are 96-well nucleic acid automatic extraction instruments, and the extraction time of one sample can be realized. The processing of 96 samples complies with the high-throughput operation requirements of biology, enabling rapid and timely response to infectious disease outbreaks, which makes traditional methods unmatched; â‘¡ The operation is simple and time-consuming, and the entire extraction process has only four steps , most of them can be completed within 36-40 minutes; â‘¢ Safe and non-toxic, no toxic reagents such as benzene and chloroform in traditional methods are used, and the damage to experimental operators is minimized, which fully conforms to modern environmental protection concepts; â‘£ Magnetic beads and nucleic acids The specific binding of the extracted nucleic acid results in high purity and high concentration of the extracted nucleic acid. According to the same principle as the silica membrane spin column, the superparamagnetic silica nano-magnetic beads are prepared after the surface of the superparamagnetic nanoparticles is modified and modified by nanotechnology. The magnetic beads can specifically recognize and efficiently bind to nucleic acid molecules on a microscopic interface. Using the superparamagnetic properties of silica-coated nanomagnetic microspheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, samples from blood, animal tissues, food, pathogenic microorganisms, etc. The isolated DNA and RNA can be used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, molecular biology research and other fields. Magnetic bead nucleic acid extraction can generally be divided into four steps: lysis-binding-washing-elution. Genetic testing will surely become a new symbol of the development of the biological industry. The emergence of high-throughput, automated nucleic acid extraction methods will reduce the labor cost of genetic testing, make large-scale testing a reality, and make it possible for genetic testing to reach ordinary people.
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