Nano-flow analysis method for cancer vesicle markers

In recent years, the rapid development of extracellular vesicles in the field of EVs research has attracted the attention of many scientists and clinicians. In particular, those who study cancer biology are eager to further explore extracellular vesicles in the diagnosis and treatment of cancer. The potential value.
A few days ago, the first study by Canadian scientist Paproski RJ et al. confirmed that the absolute release and identification of the release of extracellular vesicles by using ultra-sensitive nanofluids (Fig. 1) revealed that the use of ultrasound stimulation can be significant with nanodroplets. Enhancing the release of EVs from tumor cells allows tumor cells to release enough EVs into the bloodstream. At the same time, in these extracellular vesicle EVs, Paproski RJ and colleagues successfully detected tumor-associated proteins, DNA, mRNA and miRNA. The results of this study provide significant diagnostic value for non-invasive detection and genetic phenotypic analysis of tumors.

Figure 1: Nanoflow analysis for detection of HT1080-GFP cell-derived microvesicles

Introduction to Apogee Ultra-sensitive Nano Flow Cytometry:

The blank ultra-sensitive nano flow cytometer is developed by the British Apogee Flow manufacturer. Its unique optical design makes it possible to detect small particles of at least 80 nm and has a resolution of 10 nm, making it the only detectable less than 100 nm on the market. Flow cytometry of large and small particles. The technology of the British Apogee Flow was widely used by the US military for rapid microbiological testing on the battlefield. At the 2017 CYTO streaming conference, the excellent performance of Apogee Puls, and many scientists have shown that the Apogee ultra-sensitive nano flow cytometer is used for extracellular capsules. The European National Metrology Research Association has rated it as the most sensitive analytical system for microvesicle detection applications. This shows that this new technology has brought many advantages in small particle detection.