Regardless of whether it is a suspension-grown cell or an adherent-grown cell, the process type can be divided into the following four types according to the operation method:
First, batch culture
It means that the cells and the culture are added to the culture reactor at one time, and the cell growth and product formation are carried out simultaneously, and after a period of reaction, the entire reaction system is taken out. This kind of culture, the environment of cell growth is constantly changing, such as the continuous reduction of nutrients, the increase of metabolic inhibitors such as lactic acid, can not make the cells under an optimal condition, so it is limited in the application process. .
Second, flow-through training
In view of the shortcomings of batch culture, fresh culture medium is continuously added during the reaction to allow the cells to continue to grow and produce products, and the reaction system is taken out after the reaction is completed. It can prevent cell and metabolic products and the lack of certain nutrients to inhibit cells.
Third, semi-continuous culture
Also referred to as repeated batch culture, it means that in the batch culture process, the culture is continuously taken out, and each time a new culture solution is added, and then batch operation is performed. The difference between it and fed-batch culture is that the volume of the fed-batch culture is increasing, while the volume of the semi-continuous culture is kept constant.
Fourth, continuous cultivation
After the cells and the culture solution are added to the reactor together, the perfusion culture method is used to continuously discharge the used medium, and at the same time, the fresh culture medium is continuously added, and the cells are generally not output, so that the cells are in a constant and optimized state. Growth and product formation in the environment, but the number of cells fluctuates. If the same amount of cells as the culture medium is taken out at the same time, it is called continuous-flow culture, which is stable in the true internal environment, and there is no fluctuation in the amount of nutrients, metabolites and cells. This method is applicable to suspension cells and cells grown on microcarriers.
In these processes, continuous culture is the best type, so that the system-optimized environment conforms to the physiological and metabolic laws of cells, which is beneficial to cell growth, proliferation and product formation, but also has some disadvantages, such as large consumption of culture medium. The operation process is complicated, and the pollution opportunity is increased.
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