First, broodstock breeding broodstock cultivation pool area of ​​2 acres, water depth of 1.5 meters, the bottom flat, the pool has a certain degree of natural slope, fresh water, convenient water, open environment, good lighting conditions. Choose two or more instars, adults with good sexual maturity, large individuals, robust physique, no disease, no injuries, and brightly colored adults to be used as broodstock. Acre stocking 800-1000. During the broodstock incubation, when the water temperature is above 18°C, the ponds are filled with water three times a week. 15 days before oxytocin injection, water was injected once a day for 2 hours each time to stimulate gonad development of broodstock. Feed chopped, high quality chilled fish every morning and evening to intensify cultivation. Fish ponds will be diluted with 0.2 mg/L Quanchipu once a month.
Second, pre-production preparation 1, the spawning pool will be an area of ​​10.6 square meters, a water depth of 30 cm rectangular cement pool is divided into a number of small pools, set up shading mesh cloth, shading rate of 60% -70%. Before oxysterone production, disinfection of the pool water is performed first, and 0.5 g of copper sulfate, 0.2 g of ferrous sulfate, 1-2 g of furazolidone, and 1-2 g of oxytetracycline are soaked in each cubic meter of water for 24 hours.
2. The hatchery pool shares 4 concrete pools, each 6.6 square meters, with a water depth of 50 centimeters. Disinfect the pool before hatching. The method is the same as the spawning pool. The top of the pool should be shaded to avoid direct sunlight and keep the water temperature stable, and there is an aerator.
3. The source of water for production and incubation is from the spare fish pond water, with fresh water and sufficient dissolved oxygen.
4. Production of artificial fish nests A layer of 30 cm 40 cm 60 mesh fishnet is first laid on the bottom of the spawning tank. This mesh is semi-floating and pressed with small stones at the bottom of the spawning tank. Lay the shade cloth on the water surface. Sunfish eggs will adhere to the mesh.
Third, artificial labor
1. Selection of mature broodstock Choose a fish that is over 2 years old and whose abdomen expands and is soft and elastic. The abdomen is black and green, males, light pressure on the abdomen testis, genital hole milk white semen out, semen into the water that is scattered, indicating a good maturity; if the semen is too thick to enter the water does not spread, indicating poor maturity; if the semen is too thin, The ribbon is yellow, indicating maturity. The abdomen white is a female fish, and the lower abdomen is selected to be soft and elastic, with a clear outline of the ovaries, and the erythrocythma dehiscences for the female broodstock. The proportion of males to females is 1:1. The average body weight of each broodstock in this test is 152 grams.
2, oxytocin injection bred fish production using the second injection method. For the first injection, females were injected with 500 IU of chorionic gonadotropin per kilogram and 1 mg of pituitary pituitary. The male dose was halved and 0.5 ml per tail was injected. After 24 hours, a second injection was performed. The female fish was injected with 1000 IU of chorionic gonadotropin and 2 mg of pituitary pituitary per kilogram, and the male fish was halved in dose and 0.5 ml per tail was injected. 28 hours after the second injection of oxytocin, the broodstock began to spawn and fertilize. After 6 days of spawning, the eggs were evenly distributed on the net cloth.
Fourth, artificial hatching
1. Move eggs from the spawning pool to the hatching pool. The temperature difference between the two pools should not exceed 2°C. If the temperature exceeds 2°C, use the water in the hatching pool to gently sprinke the eggs on the mesh. To prevent the eggs from being washed off the mesh, after a period of time, the eggs can be adapted to the water temperature in the hatchery and they can be placed in the hatchery hatch. When incubating, allow the mesh to float in the water so that the fry can sink to the bottom when the fish fry.
2. Incubation and management The hatchery should maintain a slight water flow condition, and also need to open an aerator for 4 hours every day to maintain a high dissolved oxygen state. When the water temperature is 22-30°C, the seedlings are hatched after 36-50 hours. After the fry hatches, the mesh should be removed and microfluidic and aerated. When it was hatched, the seedlings did not eat at the end of the sink and they digested the yolk sac as nutrients. After 2 days, the fry floated to the surface and began to feed.
V. Spawning and emergence
1. Spawning After May 1st, 2001, some broodstock began to lay eggs, and each pair of broodstock spawned 1 nest until the broodstock laid on May 8 and lasted for 6 days. 15 nests.
2. During spawning and spawning, five litters were randomly sampled to calculate the number of eggs per litter, ie, about 7,000 eggs per female were spawned, and 15 females totaled about 105,000 eggs. Calculating the fertilization rate was 86%, the number of eggs per litter was 6,020, and the total number of fertilized eggs was 90,000. The fertilized eggs hatched after 39-42 hours. About 5320 fry were hatched from each litter and about 78,000 fry were hatched. The hatching rate reached 86.8%. It was raised to 2-4 cm for sale. A total of about 60,000 2-4 cm seedlings were sold, and the survival rate was 77%.
6. Analysis and Discussion Generally speaking, March-October is the breeding season of the blue snapper sunfish, and April-August is the breeding boom of the blue snapper sunfish. The author chose to conduct an artificial propagation experiment in May, and the oxytocin production is relatively easy. Practice has shown that broodstock is not concentrated in spawning a day, from the beginning of spawning to the end of spawning, for 6 days, broodstock spawn every day. The results showed that the development of gonads was not synchronous and spawning was sequential, which was similar to the breeding habits of California salmon. To increase the hatching rate, the main concern is that in the hatching process, the hatching pool always maintains a micro-flowing state; daily use of an aerator increases oxygen for at least 10 hours; and the eggs in the mesh cloth are in a semi-floating state and do not sink to the bottom of the pool.
Second, pre-production preparation 1, the spawning pool will be an area of ​​10.6 square meters, a water depth of 30 cm rectangular cement pool is divided into a number of small pools, set up shading mesh cloth, shading rate of 60% -70%. Before oxysterone production, disinfection of the pool water is performed first, and 0.5 g of copper sulfate, 0.2 g of ferrous sulfate, 1-2 g of furazolidone, and 1-2 g of oxytetracycline are soaked in each cubic meter of water for 24 hours.
2. The hatchery pool shares 4 concrete pools, each 6.6 square meters, with a water depth of 50 centimeters. Disinfect the pool before hatching. The method is the same as the spawning pool. The top of the pool should be shaded to avoid direct sunlight and keep the water temperature stable, and there is an aerator.
3. The source of water for production and incubation is from the spare fish pond water, with fresh water and sufficient dissolved oxygen.
4. Production of artificial fish nests A layer of 30 cm 40 cm 60 mesh fishnet is first laid on the bottom of the spawning tank. This mesh is semi-floating and pressed with small stones at the bottom of the spawning tank. Lay the shade cloth on the water surface. Sunfish eggs will adhere to the mesh.
Third, artificial labor
1. Selection of mature broodstock Choose a fish that is over 2 years old and whose abdomen expands and is soft and elastic. The abdomen is black and green, males, light pressure on the abdomen testis, genital hole milk white semen out, semen into the water that is scattered, indicating a good maturity; if the semen is too thick to enter the water does not spread, indicating poor maturity; if the semen is too thin, The ribbon is yellow, indicating maturity. The abdomen white is a female fish, and the lower abdomen is selected to be soft and elastic, with a clear outline of the ovaries, and the erythrocythma dehiscences for the female broodstock. The proportion of males to females is 1:1. The average body weight of each broodstock in this test is 152 grams.
2, oxytocin injection bred fish production using the second injection method. For the first injection, females were injected with 500 IU of chorionic gonadotropin per kilogram and 1 mg of pituitary pituitary. The male dose was halved and 0.5 ml per tail was injected. After 24 hours, a second injection was performed. The female fish was injected with 1000 IU of chorionic gonadotropin and 2 mg of pituitary pituitary per kilogram, and the male fish was halved in dose and 0.5 ml per tail was injected. 28 hours after the second injection of oxytocin, the broodstock began to spawn and fertilize. After 6 days of spawning, the eggs were evenly distributed on the net cloth.
Fourth, artificial hatching
1. Move eggs from the spawning pool to the hatching pool. The temperature difference between the two pools should not exceed 2°C. If the temperature exceeds 2°C, use the water in the hatching pool to gently sprinke the eggs on the mesh. To prevent the eggs from being washed off the mesh, after a period of time, the eggs can be adapted to the water temperature in the hatchery and they can be placed in the hatchery hatch. When incubating, allow the mesh to float in the water so that the fry can sink to the bottom when the fish fry.
2. Incubation and management The hatchery should maintain a slight water flow condition, and also need to open an aerator for 4 hours every day to maintain a high dissolved oxygen state. When the water temperature is 22-30°C, the seedlings are hatched after 36-50 hours. After the fry hatches, the mesh should be removed and microfluidic and aerated. When it was hatched, the seedlings did not eat at the end of the sink and they digested the yolk sac as nutrients. After 2 days, the fry floated to the surface and began to feed.
V. Spawning and emergence
1. Spawning After May 1st, 2001, some broodstock began to lay eggs, and each pair of broodstock spawned 1 nest until the broodstock laid on May 8 and lasted for 6 days. 15 nests.
2. During spawning and spawning, five litters were randomly sampled to calculate the number of eggs per litter, ie, about 7,000 eggs per female were spawned, and 15 females totaled about 105,000 eggs. Calculating the fertilization rate was 86%, the number of eggs per litter was 6,020, and the total number of fertilized eggs was 90,000. The fertilized eggs hatched after 39-42 hours. About 5320 fry were hatched from each litter and about 78,000 fry were hatched. The hatching rate reached 86.8%. It was raised to 2-4 cm for sale. A total of about 60,000 2-4 cm seedlings were sold, and the survival rate was 77%.
6. Analysis and Discussion Generally speaking, March-October is the breeding season of the blue snapper sunfish, and April-August is the breeding boom of the blue snapper sunfish. The author chose to conduct an artificial propagation experiment in May, and the oxytocin production is relatively easy. Practice has shown that broodstock is not concentrated in spawning a day, from the beginning of spawning to the end of spawning, for 6 days, broodstock spawn every day. The results showed that the development of gonads was not synchronous and spawning was sequential, which was similar to the breeding habits of California salmon. To increase the hatching rate, the main concern is that in the hatching process, the hatching pool always maintains a micro-flowing state; daily use of an aerator increases oxygen for at least 10 hours; and the eggs in the mesh cloth are in a semi-floating state and do not sink to the bottom of the pool.
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