The microscope is one of the greatest inventions of mankind in the 20th century. Before it was invented, humans' perceptions of the world around them were limited to the naked eye, or by hand-held lenses to help the naked eye see what they saw.
The microscope presents a whole new world in the human eye. For the first time, people have seen hundreds of "new" tiny animals and plants, as well as internal structures from the human body to plant fibers. Microscopy also helps scientists discover new species and help doctors treat diseases.
Microscopes have gradually become the most commonly used analytical instruments for researchers, but how to choose a microscope, cost-effective, and complete functions are often a headache for researchers. Today, Xiaobian will talk to you about how to choose the right one. Microscope.
First, what kind of microscope should I use to understand what type of sample?
Microscopes are divided into observational samples and different functions: upright microscope, inverted microscope, volume microscope, fluorescence microscope, phase contrast microscope, polarized light microscope, metallographic microscope, laser confocal microscope and so on. The upright microscope is more suitable for observing the slides, the inverted microscope is more suitable for the observation of the culture dish, the phase contrast microscope is more suitable for living cells and unstained biological specimens, the fluorescence microscope is suitable for autofluorescence and the induction of fluorescent specimen imaging and the like. Echo Revolve is an inverted inverted fluorescence microscope: easy to switch between positive and negative, reducing the need of two microscopes separately, saving space and cost, can complete bright field, phase difference and fluorescence, one instrument can meet various functions; Ipad pro is a perfect substitute for traditional eyepieces: super High-resolution Retina retina touch screen design with full field of view to avoid fatigue and strain; preset operation software app: streamline workflow, cloud sharing pictures.
Second, the comfort of the operating experience?
Now the comfort of the microscope experience is a big selling point of the microscope. The picture is synchronized, the screen is displayed, and the saved pictures are directly modified and edited, which is very helpful for the liberation experimenter. Revolve uses the Retina retina screen instead of the traditional eyepiece; full field of view: the same as the human eye sees through the traditional eyepiece; no vignetting (blurred edge of the field of view); avoid eye fatigue, muscle strain. At the same time, Revolve is inverting the integrated microscope for real-time projection display, which can simultaneously observe the experimental results, which is convenient and time-sensitive.
Third, what effect do you want, how many times to enlarge?
Eukaryotic cells: 10-100 μm; prokaryotic cells: 1-10 μm; nuclei: about 7 cm; mitochondria: 0.5-1 μm; most viruses: 20-100 nm. Optical microscopes can be distinguished by a minimum of 200 nm; smaller mycoplasmas and viruses need to be observed using an electron microscope. Total optical magnification = magnification of the eyepiece X objective magnification, digital total magnification = eyepiece magnification X objective X digital magnification. So choose the right microscope and configuration based on the size of the sample you are looking at.
Fourth, the quality of the optical system?
Resolving power is the ability to observe the details of the sample and the speed at which the image is resolved - another important factor to consider. The objective lens is important in the optical system. The higher the numerical aperture (NA) of the objective lens, the stronger the resolution. The level of detail and budget that the user needs to see, select the appropriate objective lens for the numerical aperture (NA). In addition to the numerical aperture (NA), it is also necessary to evaluate the degree of correction of the spherical aberration and chromatic aberration by the microscope objective. Choose the best objective in your budget. The quality of the objective lens will directly affect the quality of the data, so this is a very important factor to consider.
There are many types and functions of microscopes available for scientists to choose from. Sometimes, the choice may be one-sided. The best way is to do research based on your needs, compare different equipment, and evaluate current needs and future plans.
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