Review on Separation and Purification of Small Molecule Samples by Column Chromatography

Lisui Technology (Suzhou) Co., Ltd. is committed to the design and production of liquid phase purification systems from laboratory to industrial production level. Customized different liquid purification equipment is designed according to different sample purification requirements. Lisui Technology also develops a purification process for customers to explore specific drug samples, and designs liquid purification equipment according to the process.

At present, there are hundreds of chemicals purified and related to the technology, and there are dozens of types of fillers and purification methods. At present, there are new and more commonly used methods: high efficiency normal phase silica gel column method, reversed phase C18 high efficiency DAC column chromatography, macroporous adsorption resin method, high performance resin purification method, DEAE cellulose column chromatography.

High-efficiency normal phase silica gel column chromatography is a unique purification method developed by Lisui Technology. It is very practical for many small-polarity small-molecule samples that are difficult to separate. This method is simple and easy, not only can easily separate the difficult-to-separate samples, but also the amount of solvent used is much less than that of ordinary silica gel column chromatography. The purification time is greatly shortened, and the filler can be reused hundreds of times without Each time the purification is renewed, the packing time is saved. For the pilot or industrial production process, the repeatability is very good, and most of the samples do not need to be mixed with silica gel, only need to be dissolved and then wet loaded. The samples purified by Lisui Technology using this method are: butylphthalide, entecavir, voglibose, podophyllotoxin, reboxetine mesylate, lacidipine, rimantadine hydrochloride, dextromethorphan hydrobromide , cefprozil, salmeterol, nitrofurazone, levothyroxine sodium, fondaparin sodium, paclitaxel, and the like.

Reversed phase C18 high efficiency DAC column chromatography is a relatively common separation method. The method is quick, the purification effect is good, and the purity is high. The samples purified by Lisui Technology using this method include thymosin, desmopressin acetate, treprostin, glutathione, calcitriol and the like.

The macroporous adsorption resin method has always been a method favored by researchers. This method is very commonly used in the isolation and purification of natural product plants. Lisui Technology uses this method to separate and purify samples: total glucosides of paeony, natamycin and so on.

High-efficiency resin purification method is an increasingly popular filler in recent years. This method is simple in operation, the filler can be reused many times, the separation effect is good, and the purified sample has high purity and saves solvent. This method can be used to replace C18 packing in many cases, which can save costs. The filler commonly used by Lisui Technology is MCI GEL CHP20P resin. Lisui Technology uses this method to separate and purify samples: conjugated to estrogen, vancomycin, spiramycin, and the like.

DEAE cellulose column chromatography is mostly used for the separation and purification of proteins and polysaccharides. This method is commonly used by scholars. Combined with Lisui Technology's Refractive Index Detector and ELSD Evaporative Light Scattering Detector, it can effectively detect the elution of samples online during the purification process, providing a simple and easy way for scholars. The method saves a lot of time and testing costs. Lisui Technology uses the filler to separate and purify samples: pepsin, kelp sulfate polysaccharide, and the like.