How to extend the life of the LC column ?
1. Use the "Guard Column" to extend the life of the column.
Simply put, the “guard column†is a very short column that fills the same packing as the liquid column used. Proper use of the “Guard Column†installed in front of the LC column can block substances that can easily damage the LC column. And to achieve the effect of extending the life of the column. If you use the "guard column" improperly. Will have the opposite effect. Because during the use of the guard column. If the pollution reaches a certain level and is not replaced in time, the accumulated debris will likely rush into the column, which will cause damage to the column. therefore. When using the "guard column", the most important thing is to know when to change the guard column. This change must be replaced immediately.
2. Choose a high quality column and pay attention to the method of use; it can extend the service life of the column.
It is generally believed that the quality of the same brand and the same type of column are the same. This is true for manufacturers with strict quality management. However, if the quality management of the manufacturer is not strict enough, even the quality of the new column of the same batch number will be different. Therefore, when selecting a column, you must choose a high-quality column that is strictly managed. We know that any column will change after it has been used. Especially when used under strong acid or strong base conditions, the column is more susceptible to changes.
Therefore, columns with a wide pH range should be used as much as possible. When developing new analytical methods, many experts recommend not using old columns. Because the column is used. Columnarity will change; as a result, new analytical methods developed using old columns are likely to be used when new column analysis of the same brand is used. A situation where the intended effect cannot be achieved. In general. A new column of good quality is used when developing new analytical methods; and a pillar is best used in an analytical method; the column life can be extended.
3. Flush the column regularly to extend the life of the column.
We know that there will always be some impurities accumulating in the column after the column has been used for a while. These impurities do not come out with the mobile phase, and naturally will gradually affect the quality of the column. These impurities appear on subsequent analytical spectra. It causes strange peaks and baseline inequality.
The simple solution is to flush with a strong mobile phase. It is best to rinse after each analysis of a product. If a buffer is used for the analysis, it is best to replace the buffer with water. The buffer solution is washed with a mobile phase using a mixed solution of organic and water. It is then rinsed with 100% organic solvent. If it is not washed first with a mixture of organic and water, and washed directly with 100% organic solvent, the buffer is likely to precipitate and damage the quality of the column. It is sufficient to use a 10-20 column volume to rinse.
Experts recommend that the conditions for 100% organic solvent rinsing be added to the end of the analytical procedure, so that it is rinsed with 100% solvent per day before starting. Generally, the silica gel column can be backwashed, that is, the column is reversed and rinsed before flushing. Before using the backwashing treatment, it is best to refer to the maintenance instructions for the column. So to really protect the column and extend the life of the column, it is best to use a "guard column" and periodically rinse the column.
Beijing Bio- Wowei Biotechnology Co., Ltd. (biobw) is a high-tech company specializing in biotechnology research and extensive application and promotion in Beijing. It is located in the Jiantai Street of Fengtai District, a Beijing-based enterprise. The promotion and application of biotechnology is earlier than 2011. At the beginning, the company was established in January 2013. The company mainly provides chromatographic consumables, chromatography equipment, solid phase extraction equipment, chemical reagents, sample bottles, xenon lamps, standard products, microbial technology, strain preservation services and ATCC product agents and Other Products and services.
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1. Use the "Guard Column" to extend the life of the column.
Simply put, the “guard column†is a very short column that fills the same packing as the liquid column used. Proper use of the “Guard Column†installed in front of the LC column can block substances that can easily damage the LC column. And to achieve the effect of extending the life of the column. If you use the "guard column" improperly. Will have the opposite effect. Because during the use of the guard column. If the pollution reaches a certain level and is not replaced in time, the accumulated debris will likely rush into the column, which will cause damage to the column. therefore. When using the "guard column", the most important thing is to know when to change the guard column. This change must be replaced immediately.
2. Choose a high quality column and pay attention to the method of use; it can extend the service life of the column.
It is generally believed that the quality of the same brand and the same type of column are the same. This is true for manufacturers with strict quality management. However, if the quality management of the manufacturer is not strict enough, even the quality of the new column of the same batch number will be different. Therefore, when selecting a column, you must choose a high-quality column that is strictly managed. We know that any column will change after it has been used. Especially when used under strong acid or strong base conditions, the column is more susceptible to changes.
Therefore, columns with a wide pH range should be used as much as possible. When developing new analytical methods, many experts recommend not using old columns. Because the column is used. Columnarity will change; as a result, new analytical methods developed using old columns are likely to be used when new column analysis of the same brand is used. A situation where the intended effect cannot be achieved. In general. A new column of good quality is used when developing new analytical methods; and a pillar is best used in an analytical method; the column life can be extended.
3. Flush the column regularly to extend the life of the column.
We know that there will always be some impurities accumulating in the column after the column has been used for a while. These impurities do not come out with the mobile phase, and naturally will gradually affect the quality of the column. These impurities appear on subsequent analytical spectra. It causes strange peaks and baseline inequality.
The simple solution is to flush with a strong mobile phase. It is best to rinse after each analysis of a product. If a buffer is used for the analysis, it is best to replace the buffer with water. The buffer solution is washed with a mobile phase using a mixed solution of organic and water. It is then rinsed with 100% organic solvent. If it is not washed first with a mixture of organic and water, and washed directly with 100% organic solvent, the buffer is likely to precipitate and damage the quality of the column. It is sufficient to use a 10-20 column volume to rinse.
Experts recommend that the conditions for 100% organic solvent rinsing be added to the end of the analytical procedure, so that it is rinsed with 100% solvent per day before starting. Generally, the silica gel column can be backwashed, that is, the column is reversed and rinsed before flushing. Before using the backwashing treatment, it is best to refer to the maintenance instructions for the column. So to really protect the column and extend the life of the column, it is best to use a "guard column" and periodically rinse the column.
Beijing Bio- Wowei Biotechnology Co., Ltd. (biobw) is a high-tech company specializing in biotechnology research and extensive application and promotion in Beijing. It is located in the Jiantai Street of Fengtai District, a Beijing-based enterprise. The promotion and application of biotechnology is earlier than 2011. At the beginning, the company was established in January 2013. The company mainly provides chromatographic consumables, chromatography equipment, solid phase extraction equipment, chemical reagents, sample bottles, xenon lamps, standard products, microbial technology, strain preservation services and ATCC product agents and Other Products and services.
Http://
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