1 Pretreatment <br> The process of pulverizing, mixing and shrinking the sample is called pretreatment.
Solid sample - low water content, crushed and sieved. The water content is higher, the edible portion is chopped or dried, and then sifted and sieved.
Liquid, slurry - mixing and mixing of mutually incompatible liquids - separation and re-sampling of special samples - special treatment according to experimental requirements
2 Extraction <br> Extraction - For the solid sample, the component to be tested is transferred to the extract for extraction - for the liquid sample, the partition coefficient of a component in two mutually incompatible solvents is different, from one The solvent is transferred to another solvent for extraction purposes.
3 purification <br> The process of removing impurities is called purification.
Extraction method - suitable for liquid samples, a small number of chemical methods - to change the solubility of impurities or analytes by chemical reaction, to separate them from the original system.
Chromatography—different physicochemical properties (such as solubility, adsorption capacity, charge, molecular weight, molecular polarity, and affinity) of the components in the mixture, so that the moving speed of each component on the support is different, and the concentration is concentrated. The components are separated in different zones.
4 Concentration <br> After the sample is purified and purified, the volume becomes larger and the concentration of the analyte decreases, which is not conducive to detection. Therefore, the purpose of concentration is to reduce the sample volume and increase the concentration of the analyte. Common: concentrated at atmospheric pressure, concentrated under reduced pressure, Freeze drying, nitrogen blowing concentration method
5 Nitrogen vortex purging technology <br> The device adopts nitrogen vortex rotary purging technology. The sample is purified by nitrogen at a certain temperature to obtain a good enrichment effect. The concentrator is controlled by a microprocessor to ensure automatic concentration evaporation of the sample. The gas nozzle blows out a stream of nitrogen to form a spiral flow in the concentrating tube, which reduces the momentum of the airflow and allows the solvent to volatilize uniformly without splashing.
6 Factors affecting nitrogen blowing concentration <br> Appropriate increase in temperature can increase the recovery rate of target substances. For different substances, the concentration and pressure of the concentration can be appropriately controlled by setting the parameters of the concentrator, and the concentration time can be shortened to achieve better recovery.
6.1 Effect of nitrogen flow pressure on recovery rate <br> The nitrogen flow change of the vortex nitrogen blowing concentrator is realized by adjusting the nitrogen inlet pressure, the pipe diameter is constant, and the flow rate is proportional to the pressure. The greater the pressure of the nitrogen stream, the greater the flow rate of the nitrogen stream. The nitrogen flow hits the tube wall to form a vortex. The larger the solvent contact surface area and the vortex shear force, the faster the solvent evaporates, and the constant purge of nitrogen prevents the solvent from reacting with the air.
6.2 Effect of water bath temperature on nitrogen flow <br> The concentrating tube is immersed in a water bath to control the temperature of the solution in the concentrated tube by heat transfer. Typically the bath temperature is controlled from 30 ° C to 60 ° C. The temperature setting depends on the boiling point of the solvent in the concentrating tube and the nature of the analyte. The bath temperature is generally lower than the boiling point of the solvent, otherwise the evaporation rate may be too fast and the recovery rate may be lowered. However, if the temperature is set too low, the concentration time will be too long, and a long time nitrogen purge will also cause the substance to be tested to volatilize. The boiling point and volatility of the solvent should be fully considered when setting the bath temperature. The high temperature can shorten the concentration time, avoid the target material from contacting the air for a long time, and reduce the evaporation of the target substance. However, too high temperature will cause the solvent to boil, thus reducing the recovery rate.
7Nitrogen blowing instrument <br> The nitrogen blowing instrument is mainly used for the nitrogen blowing concentration step. The basic principle is to link with the nitrogen bottle. The nitrogen gas is introduced into the aeration plate of the nitrogen blowing device through the pressure reducing valve, and then passes through the nitrogen on the aeration plate. The sample is purged for concentration purposes and heated at the bottom of the tube to accelerate solvent evaporation.
Solid sample - low water content, crushed and sieved. The water content is higher, the edible portion is chopped or dried, and then sifted and sieved.
Liquid, slurry - mixing and mixing of mutually incompatible liquids - separation and re-sampling of special samples - special treatment according to experimental requirements
2 Extraction <br> Extraction - For the solid sample, the component to be tested is transferred to the extract for extraction - for the liquid sample, the partition coefficient of a component in two mutually incompatible solvents is different, from one The solvent is transferred to another solvent for extraction purposes.
3 purification <br> The process of removing impurities is called purification.
Extraction method - suitable for liquid samples, a small number of chemical methods - to change the solubility of impurities or analytes by chemical reaction, to separate them from the original system.
Chromatography—different physicochemical properties (such as solubility, adsorption capacity, charge, molecular weight, molecular polarity, and affinity) of the components in the mixture, so that the moving speed of each component on the support is different, and the concentration is concentrated. The components are separated in different zones.
4 Concentration <br> After the sample is purified and purified, the volume becomes larger and the concentration of the analyte decreases, which is not conducive to detection. Therefore, the purpose of concentration is to reduce the sample volume and increase the concentration of the analyte. Common: concentrated at atmospheric pressure, concentrated under reduced pressure, Freeze drying, nitrogen blowing concentration method
5 Nitrogen vortex purging technology <br> The device adopts nitrogen vortex rotary purging technology. The sample is purified by nitrogen at a certain temperature to obtain a good enrichment effect. The concentrator is controlled by a microprocessor to ensure automatic concentration evaporation of the sample. The gas nozzle blows out a stream of nitrogen to form a spiral flow in the concentrating tube, which reduces the momentum of the airflow and allows the solvent to volatilize uniformly without splashing.
6 Factors affecting nitrogen blowing concentration <br> Appropriate increase in temperature can increase the recovery rate of target substances. For different substances, the concentration and pressure of the concentration can be appropriately controlled by setting the parameters of the concentrator, and the concentration time can be shortened to achieve better recovery.
6.1 Effect of nitrogen flow pressure on recovery rate <br> The nitrogen flow change of the vortex nitrogen blowing concentrator is realized by adjusting the nitrogen inlet pressure, the pipe diameter is constant, and the flow rate is proportional to the pressure. The greater the pressure of the nitrogen stream, the greater the flow rate of the nitrogen stream. The nitrogen flow hits the tube wall to form a vortex. The larger the solvent contact surface area and the vortex shear force, the faster the solvent evaporates, and the constant purge of nitrogen prevents the solvent from reacting with the air.
6.2 Effect of water bath temperature on nitrogen flow <br> The concentrating tube is immersed in a water bath to control the temperature of the solution in the concentrated tube by heat transfer. Typically the bath temperature is controlled from 30 ° C to 60 ° C. The temperature setting depends on the boiling point of the solvent in the concentrating tube and the nature of the analyte. The bath temperature is generally lower than the boiling point of the solvent, otherwise the evaporation rate may be too fast and the recovery rate may be lowered. However, if the temperature is set too low, the concentration time will be too long, and a long time nitrogen purge will also cause the substance to be tested to volatilize. The boiling point and volatility of the solvent should be fully considered when setting the bath temperature. The high temperature can shorten the concentration time, avoid the target material from contacting the air for a long time, and reduce the evaporation of the target substance. However, too high temperature will cause the solvent to boil, thus reducing the recovery rate.
7Nitrogen blowing instrument <br> The nitrogen blowing instrument is mainly used for the nitrogen blowing concentration step. The basic principle is to link with the nitrogen bottle. The nitrogen gas is introduced into the aeration plate of the nitrogen blowing device through the pressure reducing valve, and then passes through the nitrogen on the aeration plate. The sample is purged for concentration purposes and heated at the bottom of the tube to accelerate solvent evaporation.
Active Pharmaceutical Ingredients
VOLSEN is Manufacturers and suppliers of pharmaceutical raw materials,anti psychotic pharmaceutical chemicals,anti nootropics chemicals,anti coagulants bulk drugs, anti hepatitis and anti ulcerative pharmaceutical chemicals in China.
Our Major APIs Included Crude Oxiracetam which registered in KDMF, Amisulpride, Levosulpride, Rebamipide, Tenofovir Disoproxil Fumarate, Entecavir Monohydrate Micronized, Indometain, Ambroxol HCL, Bosentan Hydrate, Drospirenone, Sorafenib Tosylate, Tegaserod Maleate, Dolutegravir, Eltrombopag Olamine.Pazopanib HCL,Vilanterol,Dabrafenib,Trametinib,Axitinib,Umeclidinium Bromide, Although our APIs do not have GMP, but all productions are based on GMP environment, strictly according GMP standard.
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Taizhou Volsen Chemical Co., Ltd. , https://www.volsenchem.com